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Structural and biochemical studies of virus : host interactions in HIV-1
(University of Missouri--Columbia, 2023)
new therapeutic targets. We have previously reported that host RNA helicase A (RHA/DHX9) is recruited into HIV-1 virions and promotes virion infectivity by enhancing the processivity of RT (4). Our preliminary studies have suggested that RHA is likely...
Informatically-informed biochemical characterization of aptamer-protein complexes
(University of Missouri--Columbia, 2022)
Nucleic acid aptamers are single-stranded oligonucleotides that fold into unique three-dimensional shapes that typically bind to targets of interest with high specificity and high affinity. However, a significant limiting ...
Roles of post-Golgi vesicular trafficking components in plant growth and immunity
(University of Missouri--Columbia, 2023)
defects in the plant immune signaling pathway. For instance, I used this technique throughout Chapter 4 to investigate a preformed callose deposition defect in Arabidopsis mutant seedlings. Additionally, the quantification methodology is valuable...
Methods of partitioning, biogenesis, and selecting for natural and engineered CoA-RNA
(University of Missouri--Columbia, 2023)
of in vivo biogenesis of CoA-RNA by performing a selection under in vivo like conditions to select for RNAs capable of capping themselves with 4' phosphopantetheine (pPant) to become CoA-RNAs (CoAzymes) and RNAs which serve as the best substrate to be capped...
Roles of clathrin-coated vesicle networks in plant immunity and development
(University of Missouri--Columbia, 2018)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] For a plant cell, the plasma membrane (PM) is not only the interface with its environment but also a signaling platform. Proteins localized to the PM ...